Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping

Oh, B.-R.; Huang, N.-T.; Chen, W.; Seo, J.; Fu, J.; Kurabayashi, K.

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Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping

Oh, B.-R.;
Huang, N.-T.;
Chen, W.;
Seo, J.;
Fu, J.;
외 1명

Citations

SCOPUS

1

초록

We developed an optofluidic biosensor by integrating a microfluidic device into a Localized Surface Plasmon Resonance (LSPR) detection system to quantitatively analyze a cell-secreted cytokine, TNF-alpha. The device was designed for on-chip trapping and incubation of immune cells and subsequent detection of cytokines by a gold nanoparticle-based LSPR sensor. During the 2 hour incubation of 50,000 THP-1 cells in the microfluidic device, we observed a shift of 0.2877nm in the LSPR surface absorbance spectrum peak, which corresponds to a TNF-alpha concentration of 327ng/mL. This device enables LSPR technique for cell-secreted cytokine assay detection by scaling down the cell secretion volume. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

키워드

Biosensor; Localized surface plasmon resonance (LSPR); Optofluidic; Cells; Cytology; Emission spectroscopy; Fluidic devices; Plasmons; Surface plasmon resonance; Absorbance spectrum; Assay detection; Detection system; Immunophenotyping; Localized surface plasmon resonance; Micro-fluidic devices; Optofluidic; Optofluidic biosensor; Biosensors

제목: Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping

저자: Oh, B.-R.; Huang, N.-T.; Chen, W.; Seo, J.; Fu, J.; Kurabayashi, K.

발행일: 2013

유형: Conference Paper

저널명: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

권: 1

페이지: 92 ~ 94

언어: ENG

출판사: Chemical and Biological Microsystems Society

분량: 3 페이지

ISSN: P 0000-0000