Fabrication and characterization of optical biosensors using polymer hydrogel microparticles and enzyme-quantum dot conjugates

초록

As a first step in the development of a microanalysis device for the detection of phenol compounds. PHEMA hydrogel microparticles containing conjugates of enzyme (tyrosinase) and quantum clot were fabricated by dispersion photopolymerization and their feasibility as optical biosensors was investigated The fluorescent response of the PHEMA microparticles containing tyrosinase-quantum clot conjugates decreased with the phenol concentration clue to the fluorescence quenching effect caused by the enzyme reaction between tyrosinase and phenol The results indicate that the activities of tyrosinase and quantum clot were maintained when they were conjugated and the conjugates encapsulated in PHEMA hydrogel microparticles. In conclusion. the PHEMA microparticles containing tyrosinase-quantum dot conjugates prepared in this study have the potential to be used as optical biosensors for the detection of phenol compounds (C) 2010 Elsevier B V All rights reserved

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