Synthesis and Characterization of P(AA- co-GelMA) Hydrogel Particles for Oral Protein Drug Delivery Applications

초록

pH-Responsive and mucoadhesive hydrogel particles using acrylic acid (AA) and gelatin were synthesized via free-radical suspension polymerization. The methacrylate groups were introduced into native gelatin to make methacrylated gelatin (GelMA) capable of polymerizing with AA. In order to determine the optimal synthesis conditions for producing the well-dispersed hydrogel particles, the effects of various reaction conditions such as concentration of dispersion stabilizer, amount of continuous medium, and stirring speed on the size and dispersibility of hydrogel particles were investigated. When the stabilizer concentration, amount of continuous medium, and stirring speed increased, the dispersibility of particles was improved. The pH-sensitive swelling and release behaviors of P(AA- co-GelMA) hydrogel particles were also investigated according to various composition of AA and gelatin in the hydrogels. P(AA- co-GelMA) hydrogel particles showed low swelling ratio at a pH less than 5 but high swelling ratio at a pH greater than 5, and the swelling ratio of the hydrogel increased at a pH above 5 with AA content in the hydrogel. The P(AA- co-GelMA) hydrogel particles also presented a pH-responsive release behavior. The difference in the released amount of FITC-dextran from the hydrogel particles between pH 2.6 and pH 7.0 decreased, when the AA content in the hydrogel decreased.

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