Development of 3D skin-equivalent in a pump-less microfluidic chip

초록

Recently, development of a human skin equivalent has been under active study. Construction of a human skin equivalent requires co-culturing multiple cell types in a 3D scaffold. The 3D skin equivalent offers advantages over 2D cell culture models, improving the physiological relevance of the model. Combination of microfluidics with 3D skin models have been studied, providing a novel in vitro platform. Here, we examined 3D skin equivalent in a microfluidic chip. Immunohistochemistry and H&E staining revealed that the skin cells proliferated and differentiated in a microfluidic environment. Our study demonstrates successful on-chip culture and differentiation of 3D skin equivalent. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

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